Construction and identification of rat pcDNA3.1(+)-antisense TβRI eukaryotic expressing plasmid
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چکیده
منابع مشابه
Construction of a Eukaryotic Plasmid Encoding Bacillus anthracis Protective Antigen, a Candidate for DNA Vaccine
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Background and purpose: Activation-induced cytidine deaminase (AID) is a B-cell specific enzyme responsible for somatic hypermutation (SHM) and class switch recombination (CSR) of antibody genes within the B-cell follicle of peripheral lymphoid organs. Ectopic overexpression of the enzyme leads to mutations in non-B cells and Escherichia coli (E.coli) genes. However, induction of mutations in E...
متن کاملconstruction of a bicistronic eukaryotic vector expressing m1 and np genes of influenza virus
objective: in this study, two conserved genes (m1 and np) of influenza virus were expressed in a bicistronic vector in order to develop a universal gene based vaccine. materials and methods: plasmids m1-pires2-egfp, pires2-np were constructed by cloning the pcr products of m1 and np genes which were amplified from the a/peurto rico/8/34 (h1n1) influenza virus strain into the plasmid expression...
متن کاملDKK1 eukaryotic expression plasmid and expression product identification.
We constructed the human dickkopf 1 (DKK1) eukaryotic expression plasmid and expressed, purified, and identified its expression product. We extracted cancer cells from cervical cancer tissue, followed by extraction of mRNA. Reverse transcription-polymerase chain reaction was conducted to obtain DKK1 gene fragments. Using these fragments, we prepared the recombinant plasmid pCMV-HA2/DKK1. The re...
متن کاملConstruction and Eukaryotic Expression of Recombinant Large Hepatitis Delta Antigen
Background: Hepatitis delta virus (HDV) is a subviral human pathogen that exploits host RNA editing activity to produce two essential forms of the sole viral protein, hepatitis delta antigen (HDAg). Editing at the amber/W site of HDV antigenomic RNA leads to the production of the large form (L-HDAg), which is required for RNA packaging. Methods: In this study, PCR-based site-directed mutagen...
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ژورنال
عنوان ژورنال: World Chinese Journal of Digestology
سال: 2006
ISSN: 2219-2859
DOI: 10.11569/wcjd.v14.i1.39